Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14587] to PRPF31 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-PRPF31 antibody [EPR14587] - BSA and Azide free
See all PRPF31 primary antibodies -
Description
Rabbit monoclonal [EPR14587] to PRPF31 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250970 is the carrier-free version of ab188577.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14587 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaThis data was developed using ab188577, the same antibody clone in a different buffer formulation.
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Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/1000 dilution + Rat spleen tissue lysate at 10 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaThis data was developed using ab188577, the same antibody clone in a different buffer formulation.
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This data was developed using ab188577, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PRPF31 with ab188577 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
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This data was developed using ab188577, the same antibody clone in a different buffer formulation.Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling PRPF31 with ab188577 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
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