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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing

Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)

Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14587] to PRPF31 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-PRPF31 antibody [EPR14587] - BSA and Azide free
    See all PRPF31 primary antibodies
  • Description

    Rabbit monoclonal [EPR14587] to PRPF31 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250970 is the carrier-free version of ab188577.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14587
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    Western blot - Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    All lanes : Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using ab188577, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    Western blot - Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/1000 dilution + Rat spleen tissue lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using ab188577, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    Immunocytochemistry - Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    This data was developed using ab188577, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PRPF31 with ab188577 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
  • Flow Cytometry - Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    Flow Cytometry - Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    This data was developed using ab188577, the same antibody clone in a different buffer formulation.Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling PRPF31 with ab188577 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
  • Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)
    Anti-PRPF31 antibody [EPR14587] - BSA and Azide free (ab250970)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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