Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15398] to SAE1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-SAE1 antibody [EPR15398] - BSA and Azide free
    See all SAE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15398] to SAE1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells.

  • General notes

    Ab232630 is the carrier-free version of ab185552. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232630 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)
    Immunoprecipitation - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)

    Immunoprecipitation from 293 cells using ab185552 at 1/50 dilution.  Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.  Blocking buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185552).

  • Flow Cytometry - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)
    Flow Cytometry - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)

    Flow cytometry analysis of HeLa cells using ab185552 at 1/230 dilution.  Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution.  Fixative: 2% paraformaldehyde.  Isotype control: Rabbit monoclonal IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185552).

  • Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)
    Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)

    Immunofluorescence analysis of MCF-7 cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185552).

  • Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)
    Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)

    Immunofluorescence analysis of HeLa cells labeling SAE1 with ab185552 at 1/500 dilution.  Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.  Fixative: 4% paraformaldehyde.  Counter stain: Dapi.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185552).

  • Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)
    Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)

    Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185552).

  • Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)
    Anti-SAE1 antibody [EPR15398] - BSA and Azide free (ab232630)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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