Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAE1 antibody [EPR15397(B)] (ab185949)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15397(B)] to SAE1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SAE1 antibody [EPR15397(B)]
    See all SAE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15397(B)] to SAE1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela, Jurkat, MCF7, 293, Raw264.7 and NIH 3T3 cell lysates; Hela and MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (ab185949)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling SAE1 with ab185949 at 1/100 dilution (red). Secondary antibody is a Goat anti-rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstain: DAPI (blue).

  • Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

  • Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/10000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

  • Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (ab185949)

    Immunofluorescent analysis of -20℃ acetone-fixed HeLa cells labeling SAE1 with ab185949 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stain: Dapi (blue).

  • Anti-SAE1 antibody [EPR15397(B)] (ab185949)
    Anti-SAE1 antibody [EPR15397(B)] (ab185949)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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