Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SAE1 antibody [EPR15397(B)] (ab185949)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15397(B)] to SAE1
Suitable for: WB, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-SAE1 antibody [EPR15397(B)]
See all SAE1 primary antibodies
Description

Rabbit monoclonal [EPR15397(B)] to SAE1
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Hela, Jurkat, MCF7, 293, Raw264.7 and NIH 3T3 cell lysates; Hela and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15397(B)
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (ab185949)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling SAE1 with ab185949 at 1/100 dilution (red). Secondary antibody is a Goat anti-rabbit IgG (Alexa Fluor^®555) at 1/200 dilution. Counterstain: DAPI (blue).
Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)

All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilution

Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

Blocking buffer: 5% NFDM/TBST
Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)

Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/10000 dilution + 293 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

Blocking buffer: 5% NFDM/TBST
Western blot - Anti-SAE1 antibody [EPR15397(B)] (ab185949)

All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilution

Lane 1 : Raw264.7 cell lysate
Lane 2 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15397(B)] (ab185949)

Immunofluorescent analysis of -20℃ acetone-fixed HeLa cells labeling SAE1 with ab185949 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stain: Dapi (blue).
Anti-SAE1 antibody [EPR15397(B)] (ab185949)

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