Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SAE1 antibody [EPR15398] (ab185552)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15398] to SAE1
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-SAE1 antibody [EPR15398]
See all SAE1 primary antibodies
Description

Rabbit monoclonal [EPR15398] to SAE1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Hela, Jurkat, 293 & MCF-7 cell lysates, Hela & MCF-7 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15398
Isotype

IgG
Research areas

Western blot - Anti-SAE1 antibody [EPR15398] (ab185552)

All lanes : Anti-SAE1 antibody [EPR15398] (ab185552) at 1/10000 dilution

Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MCF-7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

Blocking buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-SAE1 antibody [EPR15398] (ab185552)

Immunoprecipitation from 293 cells using ab185552 at 1/50 dilution. Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Blocking buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-SAE1 antibody [EPR15398] (ab185552)

Flow cytometry analysis of HeLa cells using ab185552 at 1/230 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)

Immunofluorescence analysis of MCF-7 cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)

Immunofluorescence analysis of HeLa cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
Anti-SAE1 antibody [EPR15398] (ab185552)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com