Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAE1 antibody [EPR15398] (ab185552)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15398] to SAE1
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SAE1 antibody [EPR15398]
    See all SAE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15398] to SAE1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela, Jurkat, 293 & MCF-7 cell lysates, Hela & MCF-7 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SAE1 antibody [EPR15398] (ab185552)
    Western blot - Anti-SAE1 antibody [EPR15398] (ab185552)
    All lanes : Anti-SAE1 antibody [EPR15398] (ab185552) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-SAE1 antibody [EPR15398] (ab185552)
    Immunoprecipitation - Anti-SAE1 antibody [EPR15398] (ab185552)

    Immunoprecipitation from 293 cells using ab185552 at 1/50 dilution.  Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.  Blocking buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-SAE1 antibody [EPR15398] (ab185552)
    Flow Cytometry - Anti-SAE1 antibody [EPR15398] (ab185552)

    Flow cytometry analysis of HeLa cells using ab185552 at 1/230 dilution.  Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution.  Fixative: 2% paraformaldehyde.  Isotype control: Rabbit monoclonal IgG.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)
    Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)

    Immunofluorescence analysis of MCF-7 cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)
    Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)

    Immunofluorescence analysis of HeLa cells labeling SAE1 with ab185552 at 1/500 dilution.  Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.  Fixative: 4% paraformaldehyde.  Counter stain: Dapi.

  • Anti-SAE1 antibody [EPR15398] (ab185552)
    Anti-SAE1 antibody [EPR15398] (ab185552)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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