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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Sumo

Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)

Price and availability

264 681 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rat monoclonal [4D12] to Sumo 1 - BSA and Azide free (Biotin)
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Conjugation: Biotin
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin)
    See all Sumo 1 primary antibodies
  • Description

    Rat monoclonal [4D12] to Sumo 1 - BSA and Azide free (Biotin)
  • Host species

    Rat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (proprietary-tag) corresponding to Human Sumo 1.
    Database link: P63165

  • Positive control

    • HeLa total cell extract; mouse primary culture neurons; C-33A Human cervix carcinoma cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Store In the Dark.
  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    ab179907 was produced in serum-free medium and purified under mild conditions by propriety chromatography processes and conjugated with biotin. ab179907 is filter-sterilised.
  • Clonality

    Monoclonal
  • Clone number

    4D12
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Sumo
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)

    Immunofluorescent analysis of C-33A Human cervix carcinoma cells labeling Sumo 1 with ab179907 at 10 ug/ml

  • Western blot - Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)
    Western blot - Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)
    Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907) + HeLa total cell extract

    Predicted band size: 11 kDa



    An 80 kDa single and other multiple bands were observed in HeLa total cell extract at 1 ug/ml

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)

    Immunofluorescent analysis of mouse primary culture neurons labeling Sumo 1 with ab179907 (left panel) at 10 ug/ml. DNA was stained with Hoechst (right panel).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Sumo 1 antibody [4D12] - BSA and Azide free (Biotin) (ab179907)

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    Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

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