Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP298] to Sumo 1
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Sumo 1 antibody [EP298]
See all Sumo 1 primary antibodies
Description

Rabbit monoclonal [EP298] to Sumo 1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Sumo 1 aa 1-100. The exact sequence is proprietary.
Positive control
- WB- HeLa whole cell lysate (ab150035), MCF-7 whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, C6 whole cell lysate, HL-60 cell lysate and A431 cell lysate. IHC- Human papillary cell thyroid gland carcinoma tissue, Human Breast carcinoma tissue, Human Glioma tissue, Human Urinary bladder transitional carcinoma tissue and Human tonsil tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.21% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP298
Isotype

IgG
Research areas

Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Sumo 1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF-7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133352 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133352 was shown to recognize Sumo 1 when Sumo 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Sumo 1 knockout samples were subjected to SDS-PAGE. ab133352 and ab8245 (loading control to GAPDH) were diluted 1/000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical staining of paraffin-embedded human thyroid carcinoma sections labelling Sumo 1 with purified ab133352 at dilution of 1:100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/10000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF-7 (human breast carcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 5 : C6 (rat glioma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 11.5 kDa
Observed band size: 18-90 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer 5% NFDM/TBST
Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11.5 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin-embedded Human papillary cell thyroid gland carcinoma tissue labelling Sumo 1 with un-purified ab133352 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Sumo 1 antibody [EP298] (ab133352)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution

Lane 1 : Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 : Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 : Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 11.5 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin embedded Human Glioma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] (ab133352)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Sumo 1 antibody [EP298] (ab133352)

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