Anti-UBE2I / UBC9 antibody (ab227918)
Key features and details
- Rabbit polyclonal to UBE2I / UBC9
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-UBE2I / UBC9 antibody
See all UBE2I / UBC9 primary antibodies -
Description
Rabbit polyclonal to UBE2I / UBC9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Recombinant fragment within Human UBE2I/ UBC9 (internal sequence). The exact sequence is proprietary.
Database link: P63279 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse testis tissue extract. IP: HEK-293T whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-UBE2I / UBC9 antibody (ab227918)
UBE2I / UBC9 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract using 5 μg of ab227918. Western blot was performed from the immunoprecipitate using ab227918.
Lane 1: HEK-293T whole cell extract.
Lane 2: Control in HEK-293T whole cell extract.
Lane 3: ab227918 in HEK-293T whole cell extract.
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Western blot - Anti-UBE2I / UBC9 antibody (ab227918)Anti-UBE2I / UBC9 antibody (ab227918) at 1/1600 dilution + Mouse testis extract at 50 µg
Predicted band size: 18 kDa15% SDS-PAGE gel.
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Western blot - Anti-UBE2I / UBC9 antibody (ab227918)All lanes : Anti-UBE2I / UBC9 antibody (ab227918) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa15% SDS-PAGE gel.
