Advanced Glycation End Products (AGEs) Assay Kit (ab273298)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Serum
Overview
-
Product name
Advanced Glycation End Products (AGEs) Assay Kit
See all AGE kits -
Detection method
Fluorescent -
Sample type
Serum -
Assay type
Semi-quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Advanced Glycation End Products (AGEs) Activity Assay Kit (ab273298) can be used for the semi-quantitative estimation of fluorescent AGEs levels in Biological Fluids. The assay is based on the characteristic fluorescence (Ex/Em= 360/460 nm), a characteristic that is shared by almost all AGEs. The proprietary composition of the Assay Buffer specifically distinguishes between AGEs and non-oxidized proteins. The one-step assay uses oxidized Bovine Serum Albumin (AGE-BSA) as a Positive Control. BSA is used as Background Control and its fluorescence under assay conditions is defined as 1 relative fluorescence intensity in arbitrary units (AU or RFUSample/ RFUBackground).
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AGEs Assay Buffer 1 x 50ml AGEs Positive Control (10 mg/ml) 1 x 500µl BSA (50 mg/ml) 1 x 500µl -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
-
Relevance
The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure. -
Cellular localization
Cell Membrane and Secreted -
Alternative names
- Advanced glycation end products
- AGEs
Images
-
AGE and BSA standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
AGEs-Positive (AGE-BSA) fluorescence versus BSA. The assay can distinguish both species (AGEs and non-oxidized proteins).
-
AGE activity in serum samplesAGEs amount in Pooled Human Serum from healthy donors (4 µg serum protein), Human Diabetic Serum (4 µg serum protein) and Human Urine from healthy donor.