Anti-Sumo 3 antibody (ab34661)
Key features and details
- Rabbit polyclonal to Sumo 3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Sumo 3 antibody
See all Sumo 3 primary antibodies -
Description
Rabbit polyclonal to Sumo 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanSaccharomyces cerevisiaeArabidopsis thaliana
-
Immunogen
Recombinant full length protein (Human)
-
Positive control
- Recombinant human Sumo 3 protein (ab3802) can be used as a positive control in WB. Human SUMO 3
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Sumo 3 antibody (ab34661)All lanes : Anti-Sumo 3 antibody (ab34661) at 1/1000 dilution (5% non-fat dry milk in TTBS)
Lane 1 : Human GFP SUMO 3 at 1 µg with blocked for 1 hour with 5% non-fat dry milk in TTBS
Lane 2 : Human GFP SUMO 3 at 3 µg with blocked for 1 hour with 5% non-fat dry milk in TTBS
Lane 3 : Human GFP SUMO 3 at 5 µg with blocked for 1 hour with 5% non-fat dry milk in TTBS
Lane 4 : Human GFP SUMO 3 at 10 µg with blocked for 1 hour with 5% non-fat dry milk in TTBS
Secondary
All lanes : Donkey anti rabbit IgG at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11.6 kDa why is the actual band size different from the predicted?
-
Western blot - Anti-Sumo 3 antibody (ab34661)All lanes : Anti-Sumo 3 antibody (ab34661) at 1/1000 dilution (in 5% non-fat dry milk in TTBS)
Lane 1 : MW marker
Lane 2 : GFP human SUMO 3 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
Lane 3 : GFP human SUMO 1 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
Lane 4 : GFP yeast SUMO at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
Lane 5 : GFP Arabidopsis thaliana SUMO 1 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
Lane 6 : GFP Arabidopsis thaliana SUMO 2 at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
Lane 7 : GFP tomato SUMO at 5 µg with blocking for 1 hour with 5% non-fat dry milk in TTBS
Secondary
All lanes : Donkey anti-rabbit IgG at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11.6 kDa why is the actual band size different from the predicted?
