Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sumo 3 antibody [1H9L17] (ab203570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [1H9L17] to Sumo 3
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Sumo 3 antibody [1H9L17]
    See all Sumo 3 primary antibodies

  • Description

    Rabbit monoclonal [1H9L17] to Sumo 3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Sumo 3 aa 50 to the C-terminus.
    Database link: P55854

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Jurkat and U2OS cells; Human colon carcinoma tissue; Mouse brain, testis and lung tissue lysates; Rat brain and testis tissue lysates; PC12 cell lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    1H9L17

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Sumo 3 antibody [1H9L17] (ab203570)
    Western blot - Anti-Sumo 3 antibody [1H9L17] (ab203570)
    All lanes : Anti-Sumo 3 antibody [1H9L17] (ab203570) at 2 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat testis tissue lysate

    Predicted band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 3 antibody [1H9L17] (ab203570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 3 antibody [1H9L17] (ab203570)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling Sumo 3 using ab203570 at 5 μg/mL, followed by DAB detection.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 3 antibody [1H9L17] (ab203570)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 3 antibody [1H9L17] (ab203570)

    Immunofluorescent analysis of U2OS cells labeling Sumo 3 using ab203570 at 5 μg/mL (left image). Right image: sample with immunogenic phosphopeptide. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody.

  • Flow Cytometry - Anti-Sumo 3 antibody [1H9L17] (ab203570)
    Flow Cytometry - Anti-Sumo 3 antibody [1H9L17] (ab203570)

    Flow cytometric analysis of fixed and permealized Jurkat cells labeling Sumo 3 using ab203570 at 0.5 μg/test (Black) followed by secondary detection with Alexa Fluor® 488 Goat anti-Rabbit. Cells incubated without primary antibody (grey) for control. Preincubation with immunizing peptide decreased the staining (red). 

  • Anti-Sumo 3 antibody [1H9L17] (ab203570)
    Anti-Sumo 3 antibody [1H9L17] (ab203570)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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