Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Sumo 3 antibody [1H9L17] (ab203570)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [1H9L17] to Sumo 3
Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Sumo 3 antibody [1H9L17]
See all Sumo 3 primary antibodies
Description

Rabbit monoclonal [1H9L17] to Sumo 3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Sumo 3 aa 50 to the C-terminus.
Database link: P55854

Run BLAST with Run BLAST with
Positive control
- Jurkat and U2OS cells; Human colon carcinoma tissue; Mouse brain, testis and lung tissue lysates; Rat brain and testis tissue lysates; PC12 cell lysate.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

1H9L17
Isotype

IgG
Research areas

Western blot - Anti-Sumo 3 antibody [1H9L17] (ab203570)

All lanes : Anti-Sumo 3 antibody [1H9L17] (ab203570) at 2 µg/ml

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : PC12 cell lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat testis tissue lysate

Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 3 antibody [1H9L17] (ab203570)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling Sumo 3 using ab203570 at 5 μg/mL, followed by DAB detection.
Immunocytochemistry/ Immunofluorescence - Anti-Sumo 3 antibody [1H9L17] (ab203570)

Immunofluorescent analysis of U2OS cells labeling Sumo 3 using ab203570 at 5 μg/mL (left image). Right image: sample with immunogenic phosphopeptide. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody.
Flow Cytometry - Anti-Sumo 3 antibody [1H9L17] (ab203570)

Flow cytometric analysis of fixed and permealized Jurkat cells labeling Sumo 3 using ab203570 at 0.5 μg/test (Black) followed by secondary detection with Alexa Fluor® 488 Goat anti-Rabbit. Cells incubated without primary antibody (grey) for control. Preincubation with immunizing peptide decreased the staining (red).
Anti-Sumo 3 antibody [1H9L17] (ab203570)

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