Anti-Gata6 antibody (ab22600)
Key features and details
- Rabbit polyclonal to Gata6
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Gata6 antibody
See all Gata6 primary antibodies -
Description
Rabbit polyclonal to Gata6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatChickenCowHumanPigApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent.Target
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Function
Thought to be important for regulating terminal differentiation and/or proliferation. -
Tissue specificity
Expressed in heart, gut and gut-derived tissues. -
Sequence similarities
Contains 2 GATA-type zinc fingers. -
Domain
The GATA-type zinc fingers mediate interaction with LMCD1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2627 Human
- Entrez Gene: 14465 Mouse
- Entrez Gene: 397600 Pig
- Entrez Gene: 29300 Rat
- Omim: 601656 Human
- SwissProt: P43693 Chicken
- SwissProt: Q92908 Human
- SwissProt: Q61169 Mouse
see all -
Alternative names
- Gata binding factor 6 antibody
- Gata binding protein 6 antibody
- GATA-binding factor 6 antibody
see all
Images
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Western blot - Anti-Gata6 antibody (ab22600)Anti-Gata6 antibody (ab22600) at 1 µg/ml + D3 ES Cells Day 15 EB (Gata6 +ve) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Protocols
References (23)
ab22600 has been referenced in 23 publications.
- Cao Y et al. HYDIN loss-of-function inhibits GATA4 expression and enhances atrial septal defect risk. Mech Dev 162:103611 (2020). PubMed: 32376282
- Li Y et al. Derivation of porcine extraembryonic endoderm-like cells from blastocysts. Cell Prolif 53:e12782 (2020). PubMed: 32196806
- Canizo JR et al. A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos. BMC Dev Biol 19:13 (2019). PubMed: 31272387
- Benchetrit H et al. Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell 24:983-994.e7 (2019). PubMed: 31031139
- Zhang X et al. A novel chemically defined serum- and feeder-free medium for undifferentiated growth of porcine pluripotent stem cells. J Cell Physiol N/A:N/A (2019). PubMed: 30701540
Images
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Western blot - Anti-Gata6 antibody (ab22600)Anti-Gata6 antibody (ab22600) at 1 µg/ml + D3 ES Cells Day 15 EB (Gata6 +ve) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
