Anti-Gata6 antibody (ab175349)
Key features and details
- Rabbit polyclonal to Gata6
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Gata6 antibody
See all Gata6 primary antibodies -
Description
Rabbit polyclonal to Gata6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Gata6 aa 561-579. Plus synthetic peptide corresponding to Human Gata6 aa 12-31 (KRFGAAGADASDSRAFPARE)
Sequence:KYSGQDGLYIGVSLASPAE
Database link: Q92908 -
Positive control
- HeLa cells; Human stomach tissue; 293T, MCF7, K562 and 3T3L1 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Gata6 antibody (ab175349)All lanes : Anti-Gata6 antibody (ab175349) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 3T3L1 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-rabbit-HRP at 1/15000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gata6 antibody (ab175349)Immunohistochemical analysis of Human stomach tissue labeling Gata6 with ab175349 at 1/100 dilution. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Following labeling, tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.
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Immunocytochemistry/ Immunofluorescence - Anti-Gata6 antibody (ab175349)Immunofluorescent analysis of HeLa cells labeling Gata6 with ab175349 at 1/200 dilution (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Following detection with the primary antibody, the cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1/200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
