Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 90 460 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [AP1] to APLP1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-APLP1 antibody [AP1]
See all APLP1 primary antibodies
Description

Mouse monoclonal [AP1] to APLP1
Host species

Mouse

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain cortex tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue; Human cerebrum Alzheimer's disease tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

AP1
Isotype

IgG1
Research areas

Western blot - Anti-APLP1 antibody [AP1] (ab273448)

Anti-APLP1 antibody [AP1] (ab273448) at 1/1000 dilution + Mouse brain cortex tissue lysate at 20 µg

Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 72 kDa
Observed band size: 97 kDa
why is the actual band size different from the predicted?

Exposure time: 37 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on human cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448)

Immunohistochemical analysis of paraffin-embedded human cerebrum with Alzheimer's disease tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on human cerebrum with Alzheimer's disease is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on mouse cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use secondary.
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] (ab273448)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on rat cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use secondary.
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Anti-APLP1 antibody [AP1] (ab273448)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com