Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8036(2)] to Connexin 32 / GJB1
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Connexin 32 / GJB1 antibody [EPR8036(2)]
    See all Connexin 32 / GJB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8036(2)] to Connexin 32 / GJB1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle, fetal liver, MCF-7 and Human stomach lysates; Human kidney tissue; MCF-7 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8036(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)
    Western blot - Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)
    All lanes : Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : MCF-7 lysate
    Lane 4 : Human stomach lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Connexin 32 / GJB1 with ab181374 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)
    Flow Cytometry - Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)

    Flow cytometric analysis of MCF-7 cells labeling Connexin 32 / GJB1 with ab181374 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)
    Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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