Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18826-119] to DDR2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free
    See all DDR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18826-119] to DDR2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-1080, HUVEC, A-204, A375, Human stomach, ovary, lung, Mouse ovary, lung, C2C12, C6, Rat ovary and His-tagged human DDR2 recombinant protein (aa22-399).

  • General notes

    ab280369 is the carrier-free version of ab280354. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280369 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

    Lane 1 : HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 3 : A-204 (rat muscle rhabdomyosarcoma) whole cell lysate
    Lane 4 : A375 (human malignant melanoma epithelial cell) whole cell lysate
    Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 97 kDa
    Observed band size: 120-130,70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280354, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a higher sensitivity ECL substrate.

    The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).

    Negative control: MDA-MB-231 (PMID: 28147276, 29616590)

    Exposure time: 158 seconds.

     

  • Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

    Lane 1 : Human stomach tissue lysate
    Lane 2 : Human ovary tissue lysate
    Lane 3 : Human lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 97 kDa
    Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?



    This data was developed using ab280354, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).

    Exposure time: 3 minutes.

     

  • Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

    Lane 1 : Mouse ovary tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 97 kDa
    Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?



    This data was developed using ab280354, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).

    Exposure time: 3 minutes.

     

  • Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 2 : Rat ovary tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 97 kDa
    Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?



    This data was developed using ab280354, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).

    Exposure time: 3 minutes

     

  • Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

    Lane 1 : His-tagged human DDR2 recombinant protein (aa22-399)
    Lane 2 : His-tagged human DDR1 recombinant protein (aa21-416)

    Lysates/proteins at 0.03 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 97 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    This data was developed using ab280354, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody can not recognize human DDR1.

    Both recombinant proteins are made in house and extracted from HEK-293 expression systems.

    Exposure time: 8 seconds.

     

  • Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)
    Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (ab280369)

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