Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [LP34] to Cytokeratin 5 + 6 + 18 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free
    See all Cytokeratin 5 + 6 + 18 primary antibodies

  • Description

    Mouse monoclonal [LP34] to Cytokeratin 5 + 6 + 18 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skin and tonsil tissue. Human pancreatic carcinoma and bladder carcinoma tissue. WB: Human skin lysate. HeLa, MCF7 and HepG2 whole cell lysate.

  • General notes

    ab256139 is the carrier-free version of ab40.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Lane 1 : ab40 at 0.424 µg/ml
    Lanes 2-3 : Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 0.424 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L), at 1/10000 dilution
    Lanes 2-3 : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 secs; Lane 2-3: 3 secs.

    This image was produced using the same antibody clone but in a different formulation ab40, PBS, sodium azide, glycerol and BSA.

  • Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 0.424 µg/ml + Human skin lysate at 20 µg

    Secondary
    Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Observed band size: 58 kDa why is the actual band size different from the predicted?


    Exposure time: 114 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation ab40, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)

    Immunohistochemical analysis of paraffin-embedded human skin labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human skin is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab40, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on the epithelial cells of human tonsil tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab40, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human bladder carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab40, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)

    Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human pancreatic carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab40, PBS, sodium azide, glycerol and BSA.

  • Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
    Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)

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