Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [LP34] to Cytokeratin 5 + 6 + 18
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Cytokeratin 5 + 6 + 18 antibody [LP34]
See all Cytokeratin 5 + 6 + 18 primary antibodies -
Description
Mouse monoclonal [LP34] to Cytokeratin 5 + 6 + 18 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Tissue, cells or virus within Human Cytokeratin 5 + 6 + 18. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. P13647, P05783, P02538.
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Positive control
- IHC-P: Human skin and tonsil tissue. Human pancreatic carcinoma and bladder carcinoma tissue. WB: Human skin lysate. HeLa, MCF7 and HepG2 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
LP34 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 0.424 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 15 secs; Lane 2-3: 3 secs.
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Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 1/1000 dilution + Human skin lysate at 20 µg
Secondary
Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution
Observed band size: 58 kDa why is the actual band size different from the predicted?
Exposure time: 114 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human skin labeling Cytokeratin 5 + 6 + 18 with ab40 at 1/1000 dilution, followed by a ready to use secondary. Positive staining on human skin is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on the epithelial cells of human tonsil tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human bladder carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human pancreatic carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -