AMPK alpha 1 overexpression 293T lysate (whole cell) (ab94311)
Overview
-
Product name
AMPK alpha 1 overexpression 293T lysate (whole cell) -
General notes
ab94311 is a 293T cell transfected lysate in which Human AMPK alpha 1 has been transiently over-expressed using a pCMV-AMPK alpha 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
-
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
-
Background
Function: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.
Images
-
SDS-PAGE - AMPK alpha 1 overexpression 293T lysate (whole cell) (ab94311)ab94311 at 15μg/lane on an SDS-PAGE gel.
-
Western blot - AMPK alpha 1 overexpression 293T lysate (whole cell) (ab94311)All lanes : Anti-AMPK alpha 1 antibody (ab89214) at 1/500 dilution
Lane 1 : AMPK alpha 1 293T Transfected Lysate - Positive Control (ab94311)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
