Anti-Claudin 3 (phospho Y219) antibody (ab78345)
Key features and details
- Rabbit polyclonal to Claudin 3 (phospho Y219)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Claudin 3 (phospho Y219) antibody
See all Claudin 3 primary antibodies -
Description
Rabbit polyclonal to Claudin 3 (phospho Y219) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthesized phosphopeptide derived from human Claudin 3 around the phosphorylation site of tyrosine 219.
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Positive control
- Extracts form COLO cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Claudin 3 (phospho Y219) antibody (ab78345)All lanes : Anti-Claudin 3 (phospho Y219) antibody (ab78345) at 1/500 dilution
Lane 1 : Extracts from COLO cells treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from COLO cells treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
