Anti-Claudin 3 antibody (ab52231)
Key features and details
- Rabbit polyclonal to Claudin 3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Claudin 3 antibody
See all Claudin 3 primary antibodies -
Description
Rabbit polyclonal to Claudin 3 -
Host species
Rabbit -
Specificity
ab52231 detects endogenous levels of total Claudin 3 protein. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from within amino acids 181 and 220 of Human Claudin-3
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Claudin 3 antibody (ab52231)All lanes : Anti-Claudin 3 antibody (ab52231) at 1/500 dilution
Lane 1 : Extracts from HuvEc cells, untreated.
Lane 2 : Extracts from HuvEc cells, treated with the immunizing peptide.
Predicted band size: 23 kDa
Observed band size: 23 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab52231)IHC image of ab52231 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52231, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody (ab52231)ICC/IF image of ab52231 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52231, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Western blot - Anti-Claudin 3 antibody (ab52231) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Claudin 3 antibody (ab52231)
Lane 1 : Human urothelial cells (differentiated)
Lane 2 : Human urothelial cells (non-differentiated)
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : IRDye® 800-conjugated Goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 2 minutes
Blocked with 0.1% Milk for 1 hour at 22°C
