Anti-Cingulin antibody (ab117778)
Key features and details
- Rabbit polyclonal to Cingulin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cingulin antibody
See all Cingulin primary antibodies -
Description
Rabbit polyclonal to Cingulin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide, corresponding to a region within amino acids 350-400 of Human Cingulin (NP_065821.1).
-
Positive control
- Jurkat, HeLa and 293T whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab117778 was affinity purified using an epitope specific to Cingulin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Cingulin antibody (ab117778)All lanes : Anti-Cingulin antibody (ab117778) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : Jurkat whole cell lysates at 50 µg
Lane 4 : HeLa whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 136 kDa
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-Cingulin antibody (ab117778)Detection of Cingulin by Western Blot of Immunprecipitate.
ab117778 at 1µg/ml staining Cingulin in 293T whole cell lysate immunoprecipitated using ab117778 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
