Anti-Cingulin antibody (ab244406)
Key features and details
- Rabbit polyclonal to Cingulin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cingulin antibody
See all Cingulin primary antibodies -
Description
Rabbit polyclonal to Cingulin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Cingulin aa 300-450.
Database link: Q9P2M7 -
Positive control
- WB: RT4 and Caco-2 cell lysate. Human liver tissue lysate. ICC/IF: A431 cells. IHC-P: Human small intestine tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cingulin antibody (ab244406)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Cingulin (green) using ab244406 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cingulin antibody (ab244406)Paraffin-embedded human small intestine tissue stained for Cingulin using ab244406 at 1/1000 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cingulin antibody (ab244406)Paraffin-embedded human lymph node tissue stained for Cingulin using ab244406 at 1/1000 dilution in immunohistochemical analysis.
Low expression as expected.
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Western blot - Anti-Cingulin antibody (ab244406)All lanes : Anti-Cingulin antibody (ab244406) at 0.4 µg/ml
Lane 1 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
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Western blot - Anti-Cingulin antibody (ab244406)All lanes : Anti-Cingulin antibody (ab244406) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
