Anti-MGAT5 antibody (ab87977)
Key features and details
- Mouse monoclonal [3E9] to MGAT5
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-MGAT5 antibody [3E9]
See all MGAT5 primary antibodies -
Description
Mouse monoclonal [3E9] to MGAT5 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Hamster
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Immunogen
Recombinant fragment: LAEPGQSCKQ VCQESQLICE PSFFQHLNKD KDMLKYKVTC QSSELAKDIL VPSFDPKNK HCVFQGDLLL FSCAGAHPRH QRVCPCRDFI KGQVALCKD, corresponding to amino acids 642 - 739 of Human MGAT5 (NP_002401) with a proprietary tag
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Positive control
- Jurkat whole cell lysate (ab7899)
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General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
3E9 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MGAT5 antibody (ab87977) Image is courtesy of an AbReview submitted by Armen Petrosyan.Anti-MGAT5 antibody (ab87977) at 1/2000 dilution + Human RWPE prostate cells at 20 µg
Secondary
Donkey anti-mouse polyclonal HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Additional bands at: 55 kDa (possible non-specific binding), 95 kDa (possible post-translational modification)
Exposure time: 40 secondsBlocking was via 5% milk in PBST incubated for 1 hour at 22°C
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-MGAT5 antibody (ab87977)Overlay histogram showing Jurkat cells stained with ab87977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87977, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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Western blot - Anti-MGAT5 antibody (ab87977)Anti-MGAT5 antibody (ab87977) at 1 µg/ml + recombinant protein fragment with a proprietary tag (the immunogen) at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 85 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?The recombinant protein used as the immunogen has a predicted size of 37 kDa.
This image was generated using the ascites version of the product.
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Western blot - Anti-MGAT5 antibody (ab87977)Anti-MGAT5 antibody (ab87977) at 1 µg/ml + jurkat cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 85 kDaThis image was generated using the ascites version of the product.
