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Signal Transduction Cytoskeleton / ECM Cell Adhesion Tight Junctions

Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13414] to Zonula occludens protein 3/ZO3 - Plasma Membrane Marker
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker
    See all Zonula occludens protein 3/ZO3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13414] to Zonula occludens protein 3/ZO3 - Plasma Membrane Marker
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Caco2 and MCF7 cell line; MCF7 cells.
  • General notes

     

     

     This product was previously labelled as Zonula occludens protein 3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13414
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Tight Junctions

Images

  • Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)
    Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)
    All lanes : Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991) at 1/100000 dilution

    Lane 1 : Caco2 cell line
    Lane 2 : MCF7 cell line

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 103 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)
    Immunocytochemistry/ Immunofluorescence - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cell line staining Zonula occludens protein 3 using ab181991 at a 1/250 dilution (green). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200. Counter stain: Dapi (blue).

  • Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)
    Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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