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Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)

Key features and details

  • Mouse monoclonal [119] to HHV6AgQ1
  • Suitable for: Flow Cyt (Intra), ELISA, WB, ICC/IF, IP
  • Reacts with: Human herpesvirus 6
  • Isotype: IgG

Overview

  • Product name

    Anti-HHV6AgQ1 antibody [119] - BSA and Azide free

  • Description

    Mouse monoclonal [119] to HHV6AgQ1

  • Host species

    Mouse

  • Specificity

    ab179902 reacts with gQ1 of HHV6A and HHV6B. This antibody is not recommended for IP of HHV-6B.

  • Tested applications

    Suitable for: Flow Cyt (Intra), ELISA, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human herpesvirus 6

  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human Herpesvirus HHV6AgQ1 aa 3-422. (Expressed in E. coli).
    Sequence:

    TARLSAMKPPRSCALIFLCAFSMATAPTNATAHRRAGTVKSTPPPEDKHS YTAKYYDKDIYFNIYEGRNSTPRRRTLSEIISKFSTSEMLSLKRVKAFVP VDENPTTTLEDIADILNYAVCDDNSCGCTIETQARIMFGDIIICVPLSAD NKGVRNFKDRIMPKGLSQILSSSLGLHLSLLYGAFGSNYNSLAYMRRLKP LTAMTAIRFCPMTTKLELRQNYKVKETLCELIVSIEILKIRNNGGQTMKT LTSFAIVRKDNDGQDWETCTRFAPVNIEDILRYKRVANDTCCRHRDVQHG RRTLESSNSWTQTQYFEPWQDIVDVYVPINDTHCPNDSYVVFETLQGFEW CSRLNKNETKNYLSSVLGFRNALFETEELMETIAMRLASQILSMVGQQGT TIRDIDPAIVSALWHSLPEN


    Database link: Q69572
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • T-cell line HSB-2 cells infected with HHV-6A; 293T cells transfected with HHV6 U100 expression plasmid. 293T cells : Flow Cyt (Intra).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
  • Concentration information loading...

  • Purity

    Proprietary Purification

  • Purification notes

    ab179902 is produced in serum-free medium and purified by proprietary chromatography procedures under mild conditions. ab179902 is 90~95% pure by SDS-PAGE and is filter-sterilised.

  • Clonality

    Monoclonal

  • Clone number

    119

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)
    Western blot - Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)
    All lanes : Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)

    Lane 1 : Non-treated
    Lane 2 : Treated with end-glycosilase
    Lane 3 : Treated with peptide N -glycosidase


    gQ1 is detected as two glycoproteins with 80 kDa and 74 kDa molecular masses

  • Immunoprecipitation - Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)
    Immunoprecipitation - Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)
    All lanes : Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)

    Lane 1 : HSB-2 cells
    Lane 2 : HSB-2 cells infected with HHV-6A GS strain
  • Immunocytochemistry/ Immunofluorescence - Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)
    Immunocytochemistry/ Immunofluorescence - Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)

    Immunofluorescent analysis of HHV6A-infected HSB-2 cells labeling HHV6 U100 using FITC-conjugated ab179902 at 1/200 dilution. The infected cells were harvested 3 days postinfection, fixed in cold acetone.

  • Flow Cytometry - Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)
    Flow Cytometry - Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)

    293T cells were transfected with gQ1 expression plasmid. The cells were harvested on second day postinfection, fixed with 4% PFA, permeabilized with 0.1% Triton-X100, incubated with anti-gQ1 antibody (119) and then with FITC-conjugated anti-mouse IgG antibody.Histograms show fluorescence intensities measured in arbitary units on a log scale (x axis) and relative cell numbers on a linear scale (y axis).Black line is control and red line is gQ1 introduced cells.

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