Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14138] to ATP1A3 - BSA and Azide free
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free
    See all ATP1A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14138] to ATP1A3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Mouse primary neuron cells WB: rat and mouse brain lysates, unboiled.

  • General notes

    ab250624 is the carrier-free version of ab182571.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182571).

  • Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)
    Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)

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