Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)
![Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)](https://www.abcam.com/ps/products/250/ab250624/Images/ab250624-1-anti-atp1a3-antibody-epr14138-immunocytochemistry-neuron-mouse.jpg "Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14138] to ATP1A3 - BSA and Azide free
- Suitable for: IP, WB, ICC/IF
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free
See all ATP1A3 primary antibodies -
Description
Rabbit monoclonal [EPR14138] to ATP1A3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Mouse primary neuron cells WB: rat and mouse brain lysates, unboiled.
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General notes
ab250624 is the carrier-free version of ab182571.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14138 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)
Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182571).
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![Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)](https://www.abcam.com/ps/products/250/ab250624/Images/ab250624-2-benefits-of-recombinant-antibodies.png)
Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)
