Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µg 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Claudin18 antibody [34H14L15] (ab203563)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [34H14L15] to Claudin18
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Claudin18 antibody [34H14L15]
See all Claudin18 primary antibodies
Description

Rabbit monoclonal [34H14L15] to Claudin18
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Claudin18 aa 200 to the C-terminus.
Database link: P56856

Run BLAST with Run BLAST with
Positive control
- WB: Mouse lung lysate. Rat heart lysate. A431 and A549 lysates. IHC-P: Human stomach and squamous lung carcimona tissues. ICC/IF: Caco-2 cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

34H14L15
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (ab203563)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Claudin18 with ab203563 at 2 µg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Claudin18 antibody [34H14L15] (ab203563)

All lanes : Anti-Claudin18 antibody [34H14L15] (ab203563) at 1/1000 dilution

Lane 1 : Rat heart lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) cell lysate

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/5000 dilution

Predicted band size: 28 kDa

4-12% Bis-Tris gel
Immunocytochemistry/ Immunofluorescence - Anti-Claudin18 antibody [34H14L15] (ab203563)

Immunofluorescent analysis of Caco-2 (Human colorectal adenocarcinoma cell line) cells (4% paraformaldehyde-fixed) labeling Claudin18 with ab203563 at 1/500 dilution followed with an Alexa Fluor^® 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. Panel c is a merged image showing cell junction localization and panel d is a control without primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (ab203563)

Formalin-fixed, paraffin-embedded human squamous lung carcinoma tissue stained for Claudin18 with ab203563 at 2 µg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Claudin18 antibody [34H14L15] (ab203563)

All lanes : Anti-Claudin18 antibody [34H14L15] (ab203563) at 2 µg/ml

Lane 1 : Mouse lung lysate
Lane 2 : Mouse lung lysate with immunizing peptide

Predicted band size: 28 kDa
Anti-Claudin18 antibody [34H14L15] (ab203563)

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