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Anti-Claudin18 antibody [34H14L15] (ab203563)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [34H14L15] to Claudin18
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Claudin18 antibody [34H14L15]
    See all Claudin18 primary antibodies

  • Description

    Rabbit monoclonal [34H14L15] to Claudin18

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Claudin18 aa 200 to the C-terminus.
    Database link: P56856

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Mouse lung lysate. Rat heart lysate. A431 and A549 lysates. IHC-P: Human stomach and squamous lung carcimona tissues. ICC/IF: Caco-2 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    34H14L15

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (ab203563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (ab203563)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for Claudin18 with ab203563 at 2 µg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Claudin18 antibody [34H14L15] (ab203563)
    Western blot - Anti-Claudin18 antibody [34H14L15] (ab203563)
    All lanes : Anti-Claudin18 antibody [34H14L15] (ab203563) at 1/1000 dilution

    Lane 1 : Rat heart lysate
    Lane 2 : A431 (Human epidermoid carcinoma cell line) cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/5000 dilution

    Predicted band size: 28 kDa



    4-12% Bis-Tris gel

  • Immunocytochemistry/ Immunofluorescence - Anti-Claudin18 antibody [34H14L15] (ab203563)
    Immunocytochemistry/ Immunofluorescence - Anti-Claudin18 antibody [34H14L15] (ab203563)

    Immunofluorescent analysis of Caco-2 (Human colorectal adenocarcinoma cell line) cells (4% paraformaldehyde-fixed) labeling Claudin18 with ab203563 at 1/500 dilution followed with an Alexa Fluor® 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. Panel c is a merged image showing cell junction localization and panel d is a control without primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (ab203563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin18 antibody [34H14L15] (ab203563)

    Formalin-fixed, paraffin-embedded human squamous lung carcinoma tissue stained for Claudin18 with ab203563 at 2 µg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Claudin18 antibody [34H14L15] (ab203563)
    Western blot - Anti-Claudin18 antibody [34H14L15] (ab203563)
    All lanes : Anti-Claudin18 antibody [34H14L15] (ab203563) at 2 µg/ml

    Lane 1 : Mouse lung lysate
    Lane 2 : Mouse lung lysate with immunizing peptide

    Predicted band size: 28 kDa

  • Anti-Claudin18 antibody [34H14L15] (ab203563)
    Anti-Claudin18 antibody [34H14L15] (ab203563)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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