Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
![Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)](https://www.abcam.com/ps/products/228/ab228978/Images/ab228978-3-anti-slam-cd150-antibody-epr22904-135-immunohistochemistry-bone-marrow-mouse.jpg "Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22904-135] to SLAM / CD150
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SLAM / CD150 antibody [EPR22904-135]
See all SLAM / CD150 primary antibodies -
Description
Rabbit monoclonal [EPR22904-135] to SLAM / CD150 -
Host species
Rabbit -
Specificity
IHC-P is recommended for mouse only.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse thymus and spleen lysates; rat thymus lysate. IHC-P Mouse spleen and bone marrow tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22904-135 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Western blot - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)](https://www.abcam.com/ps/products/228/ab228978/Images/ab228978-1-anti-slam-cd150-antibody-epr22904-135-western-blot-mouse-rat.jpg)
Western blot - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)All lanes : Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978) at 1/1000 dilution
Lane 1 : Mouse thymus lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse lung lysate
Lane 4 : Rat thymus lysate
Lane 5 : Rat lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 26 seconds; Lanes 2-5: 136 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28982149).
Negative control: Lung (PMID: 11950898)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)](https://www.abcam.com/ps/products/228/ab228978/Images/ab228978-2-anti-slam-cd150-antibody-epr22904-135-immunohistochemistry-spleen-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)](https://www.abcam.com/ps/products/228/ab228978/Images/ab228978-4-benefits-of-recombinant-antibodies.png)
Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
