Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLAM / CD150 antibody [EPR22904-135] - BSA and Azide free (ab256359)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22904-135] to SLAM / CD150 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-SLAM / CD150 antibody [EPR22904-135] - BSA and Azide free
    See all SLAM / CD150 primary antibodies

  • Description

    Rabbit monoclonal [EPR22904-135] to SLAM / CD150 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IHC-P is recommended for mouse only.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus and spleen lysates; rat thymus lysate. IHC-P Mouse spleen and bone marrow tissue.

  • General notes

    Ab256359 is the carrier-free version of ab228978. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256359 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] - BSA and Azide free (ab256359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] - BSA and Azide free (ab256359)

    Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).


    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.


    The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.


     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228978).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] - BSA and Azide free (ab256359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] - BSA and Azide free (ab256359)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).


    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.


    The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.


     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228978).
  • Anti-SLAM / CD150 antibody [EPR22904-135] - BSA and Azide free (ab256359)
    Anti-SLAM / CD150 antibody [EPR22904-135] - BSA and Azide free (ab256359)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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