Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling NCOA62/SNW1 with ab70828 at 1/200 (1µg/ml). Detection: DAB.

All lanes : Anti-NCOA62/SNW1 antibody (ab70828) at 0.04 µg/ml

Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg

Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

Detection of Human NCOA62/SNW1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70828 at 3 µg/mg lysate (Lane 1). Lane 2 represents IgG IP control. Subsequent Western blot detection of NCOA62/SNW1 was performed using ab70828 at 1 µg/ml.