Anti-NCOA62/SNW1 antibody (ab70827)
Key features and details
- Rabbit polyclonal to NCOA62/SNW1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-NCOA62/SNW1 antibody
See all NCOA62/SNW1 primary antibodies -
Description
Rabbit polyclonal to NCOA62/SNW1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
-
Immunogen
Synthetic peptide corresponding to Human NCOA62/SNW1 aa 450-550.
Database link: Q13573 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA62/SNW1 antibody (ab70827)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NCOA62/SNW1 with ab70827 at 1/1000 (1µg/ml). Detection: DAB.
-
Western blot - Anti-NCOA62/SNW1 antibody (ab70827)All lanes : Anti-NCOA62/SNW1 antibody (ab70827) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 40 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
-
Immunoprecipitation - Anti-NCOA62/SNW1 antibody (ab70827)Detection of Human NCOA62/SNW1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70827 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
