Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2580(2)] to PIAS1 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free
    See all PIAS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2580(2)] to PIAS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells

  • General notes

    ab239973 is the carrier-free version of ab109388.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)
    Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PIAS1 with purified ab109388 at 1/500 dilution (4.6 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109388).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109388).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)
    OI-RD Scanning - Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109388).

  • Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)
    Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PIAS1 antibody [EPR2580(2)] - BSA and Azide free (ab239973)

© 2021 Astana Biomed Group LLP. All rights reserved.