Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2580(2)] to PIAS1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PIAS1 antibody [EPR2580(2)]
    See all PIAS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2580(2)] to PIAS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates. ICC/IF: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
    Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution

    All lanes : Recombinant Human PIAS1 protein (ab152888)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa
    Observed band size: 98 kDa
    why is the actual band size different from the predicted?



    Exposure time:

    Lane 1: 10 seconds.

    Lane 2: 1 minute.

     

    Blocking and dilution buffer: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PIAS1 with purified ab109388 at 1/500 dilution (4.6 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. 

  • Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : HEK-293 cell lysates
    Lane 4 : Daudi cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 72 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    OI-RD Scanning - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PIAS1 antibody [EPR2580(2)] (ab109388)
    Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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