Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)
![Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)](https://www.abcam.com/ps/products/259/ab259913/Images/ab259913-457452-antiKDM5C--Jarid1C--SMCX-antibody-EPR2393218-western-blot-ko-hek293t.jpg "Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23932-18] to KDM5C / Jarid1C / SMCX
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18]
See all KDM5C / Jarid1C / SMCX primary antibodies -
Description
Rabbit monoclonal [EPR23932-18] to KDM5C / Jarid1C / SMCX -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ChIP,Flow Cyt (Intra),ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, HeLa, HAP1, NCCIT.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23932-18 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)All lanes : Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution
Lane 1 : Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 :Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266252)
Lane 3 :Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266251)
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776)
Predicted band size: 175 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Lanes 1-4: Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
Lanes 1-3: ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)](https://www.abcam.com/ps/products/259/ab259913/Images/ab259913-457453-antiKDM5C--Jarid1C--SMCX-antibody-EPR2393218-western-blot-hap1.jpg)
Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)All lanes : Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/1000 dilution
Lane 1 : HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 175 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Exposure time 70 seconds
Smaller bands are non-specific signal.
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![Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)](https://www.abcam.com/ps/products/259/ab259913/Images/ab259913-457454-antiKDM5C--Jarid1C--SMCX-antibody-EPR2393218-western-blot-NCCIT.jpg)
Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913) at 1/2000 dilution + NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 175 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Exposure time 3 minutes
Smaller bands are non-specific signal.
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![Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)](https://www.abcam.com/ps/products/259/ab259913/Images/ab259913-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (ab259913)