Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574)
Key features and details
- Rabbit polyclonal to Tryptophan Hydroxylase/TPH (phospho S260)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody
See all Tryptophan Hydroxylase/TPH primary antibodies -
Description
Rabbit polyclonal to Tryptophan Hydroxylase/TPH (phospho S260) -
Host species
Rabbit -
Specificity
Ab30574 recognises Tryptophan Hydroxylase/TPH (phospho S260).
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Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide corresponding to Rat Tryptophan Hydroxylase/TPH.
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Positive control
- Rat brain stem lysate, human dorsal Raphe nucleus.
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General notes
This product was previously labelled as Tryptophan Hydroxylase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Ab30574 was affinity purified via sequential chromatography on phospho and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574)All lanes : Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574) at 1/1000 dilution
Lane 1 : rat brain stem lysate (control)
Lane 2 : rat brain stem lysate, incubated with S260 phosphopeptide (negative control)
Lane 3 : rat brain stem lysate, incubated with corresponding non phosphopeptide.
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at:
In lane 2, the labeling is specifically blocked by the S260 phosphopeptide used as antigen.
In lane 3, the corresponding nonphosphopeptide did not block the immunolabeling.
