Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Claudin 5 antibody [EPR7583] (ab131259)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7583] to Claudin 5
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-Claudin 5 antibody [EPR7583]
See all Claudin 5 primary antibodies
Description

Rabbit monoclonal [EPR7583] to Claudin 5
Host species

Rabbit
Specificity

ab131259 failed to detect target band in mouse brain tissue.

Mouse species is recommended based on WB result. This antibody is unsuitable to be used in IHC on mouse tissues.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide within Human Claudin 5 aa 200 to the C-terminus (intracellular). The exact sequence is proprietary.
Database link: O00501
Positive control
- Human fetal brain, lung squamous cell cancer and small cell lung cancer, and Mouse lung lysates; Human kidney and lung tissues.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7583
Isotype

IgG
Research areas

Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259)

All lanes : Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/1000 dilution

Lane 1 : Mouse lung tissue lysates with 5% NFDM/TBST
Lane 2 : Rat lung tissue lysates with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Predicted band size: 23 kDa
Observed band size: 20 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

We didn’t recommend rat for that ab131259 failed to detect target band in rat lung in which Claudin 5 is highly expressed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Immunohistochemical staining of paraffin embedded human colon with purified ab131259 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259)

All lanes : Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal brain lysate
Lane 2 : Human lung squamous cell cancer lysate
Lane 3 : Human small cell lung cancer lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 23 kDa
Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/5000 dilution (purified) + Human small cell lung cancer at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/5000 dilution

Predicted band size: 23 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/20000 dilution (purified) + Human lung squamous cell cancer lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/5000 dilution

Predicted band size: 23 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Unpurified ab131259 showing positive staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Unpurified ab131259 showing positive staining in Normal placenta tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Unpurified ab131259 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Unpurified ab131259 showing positive staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Claudin 5 antibody [EPR7583] (ab131259)

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