Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Brk/PTK6 antibody [EPR21051-68] (ab233391)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21051-68] to Brk/PTK6
Suitable for: WB, IP, Flow Cyt
Reacts with: Human

Product name

Anti-Brk/PTK6 antibody [EPR21051-68]
See all Brk/PTK6 primary antibodies
Description

Rabbit monoclonal [EPR21051-68] to Brk/PTK6
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, HT-29 and T-47D whole cell lysates; Human breast cancer lysate. Flow Cytometry: T-47D cells. IP: T-47D whole cell lysate.
General notes
This product was previously labelled as Brk

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21051-68
Isotype

IgG
Research areas

Western blot - Anti-Brk/PTK6 antibody [EPR21051-68] (ab233391)

All lanes : Anti-Brk/PTK6 antibody [EPR21051-68] (ab233391) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : Human breast cancer lysate
Lane 4 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 52 kDa
Observed band size: 48 kDa
why is the actual band size different from the predicted?

Exposure time : Lanes 1-3: 3 minutes; Lane 4: 75 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-Brk/PTK6 antibody [EPR21051-68] (ab233391)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized T-47D (human ductal breast epithelial tumor cell line) cell line labeling Brk/PTK6 with ab233391 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-68] (ab233391)

Brk/PTK6 was immunoprecipitated from 0.35 mg of T-47D (human ductal breast epithelial tumor cell line) whole cell lysate with ab233391 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233391 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: T-47D whole cell lysate 10 µg (Input).

Lane 2: ab233391 IP in T-47D whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233391 in T-47D whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-Brk/PTK6 antibody [EPR21051-68] (ab233391)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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