Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21051-96] to Brk/PTK6
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Brk/PTK6 antibody [EPR21051-96]
    See all Brk/PTK6 primary antibodies

  • Description

    Rabbit monoclonal [EPR21051-96] to Brk/PTK6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T-47D, MCF7, HT-29 and PC-3 whole cell lysates; Human breast cancer and skin lysates. IHC-P: Human breast cancer and colon tissues. Flow Cytometry: T-47D cells. IP: T-47D whole cell lysate.

  • General notes

     This product was previously labelled as Brk

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)
    Western blot - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)
    All lanes :

    Lane 1 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
    Lane 5 : Human skin lysate
    Lane 6 : Human breast cancer lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1: 15 seconds; Lane 2-6: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Brk/PTK6 with ab233392 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in surface epithelium of human colon (PMID: 10430081) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)
    Flow Cytometry - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized T-47D (human ductal breast epithelial tumor cell line) cell line labeling Brk/PTK6 with ab233392 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)
    Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)

    Brk/PTK6 was immunoprecipitated from 0.35 mg of T-47D (human ductal breast epithelial tumor cell line) whole cell lysate with ab233392 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233392 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: T-47D whole cell lysate 10 µg (Input). 

    Lane 2: ab233392 IP in T-47D whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233392 in T-47D whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    IgG heavy chain: PMID: 18781181

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Brk/PTK6 with ab233392 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in and membranous staining on human breast cancer cells is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)
    Anti-Brk/PTK6 antibody [EPR21051-96] (ab233392)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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