Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD62L antibody [EPR17012-35] (ab253241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17012-35] to CD62L
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD62L antibody [EPR17012-35]
    See all CD62L primary antibodies

  • Description

    Rabbit monoclonal [EPR17012-35] to CD62L

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse serum. Flow Cyt: Mouse splenocytes.

  • General notes

    ab253241 (EPR17012-35) is the detector of the ssELISA kit ab199085.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-CD62L antibody [EPR17012-35] (ab253241)
    Flow Cytometry - Anti-CD62L antibody [EPR17012-35] (ab253241)

    Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253241 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD4 and BV421-conjugated anti-CD44. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.

    Gated on viable CD4+ T cells.

  • Western blot - Anti-CD62L antibody [EPR17012-35] (ab253241)
    Western blot - Anti-CD62L antibody [EPR17012-35] (ab253241)
    All lanes : Anti-CD62L antibody [EPR17012-35] (ab253241) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse lymph node lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa
    Observed band size: 75-100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 136 seconds


    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 12165530).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-CD62L antibody [EPR17012-35] (ab253241)
    Anti-CD62L antibody [EPR17012-35] (ab253241)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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