Anti-CD62L antibody [OX85] - BSA and Azide free (ab244587)
![Anti-CD62L antibody [OX85] - BSA and Azide free (ab244587)](https://www.abcam.com/ps/products/244/ab244587/Images/ab244587-1-ab238473flow1.jpg "Anti-CD62L antibody [OX85] - BSA and Azide free (ab244587)")
Key features and details
- Mouse monoclonal [OX85] to CD62L - BSA and Azide free
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD62L antibody [OX85] - BSA and Azide free
See all CD62L primary antibodies -
Description
Mouse monoclonal [OX85] to CD62L - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt RatIHC-Fr Rat
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Immunogen
Fusion protein corresponding to Rat CD62L (extracellular). Fused at the C-terminal end to domains 3 and 4 of rat CD4.
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Positive control
- Flow Cyt: Lewis rat splenocytes. IHC-Fr: Rat Spleen
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General notes
ab244587 is the PBS only version of ab238473.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OX85 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD62L antibody [OX85] - BSA and Azide free (ab244587)
This data was generating using the same clone in a different formulation (ab238473)
Lewis rat splenocytes stained with ab238473 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab238473) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
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![Immunohistochemistry (Frozen sections) - Anti-CD62L antibody [OX85] - BSA and Azide free (ab244587)](https://www.abcam.com/ps/products/244/ab244587/Images/ab244587-365938-anticd62lox85ihcfrrtspleen.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD62L antibody [OX85] - BSA and Azide free (ab244587)This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238473).
IHC image of CD62L staining in a section of frozen normal Rat Spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238473 at 1µg/ml and ab16669 (Rabbit monoclonal [SP7] to CD3) at 1/150, to show the co-staining in naive T cells. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) and ab150080 (Goat Anti-Rabbit IgG H&L (Alexa Fluor®594) (Shown in red) 1/1000) for 1 hour at room temperature. The secondary-only control insert image is taken from an identical assay without primary antibody. DAPI was used to stain the cell nuclei (blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
