Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (ab254203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17012-27] to CD62L - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD62L antibody [EPR17012-27] - BSA and Azide free
    See all CD62L primary antibodies

  • Description

    Rabbit monoclonal [EPR17012-27] to CD62L - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Mouse splenocytes.

  • General notes

    Ab254203 is the carrier-free version of ab253240. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254203 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (ab254203)
    Flow Cytometry - Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (ab254203)

    Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253240 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with anti-CD4 conjugated with Alexa Fluor® 647 and anti-CD44 conjugated with BV421. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.

    Gated on viable CD4+ T cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253240).

  • Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (ab254203)
    Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (ab254203)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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