Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)
![Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)](https://www.abcam.com/ps/products/273/ab273649/Images/ab273649-394442-anti-anxa3-antibody-wb-hepg2-hela-ht29-mcf7-cells.jpg "Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)")
Key features and details
- Mouse monoclonal [OTI5G4] to Annexin A3/ANXA3 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free
See all Annexin A3/ANXA3 primary antibodies -
Description
Mouse monoclonal [OTI5G4] to Annexin A3/ANXA3 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Dog, Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human Annexin A3/ANXA3.
Database link: P12429 -
Positive control
- WB: A3/ANXA3 transfected HEK-293T lysate; HepG2, HeLa, HT29 and MCF7 cell lysate; IHC-P: Human lung tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
OTI5G4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)All lanes : Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK (canine kidney cell line) whole cell lysate
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 36 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)](https://www.abcam.com/ps/products/273/ab273649/Images/ab273649-394441-anti-anxa3-antibody-ihc-human-prostate-tissue.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)Immunohistochemical analysis of paraffin-embedded human prostate tissue staining of ANXA3 with a mouse monoclonal
Annexin A3/ANXA3 antibody [OTI5G4].This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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![Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)](https://www.abcam.com/ps/products/273/ab273649/Images/ab273649-394443-anti-anxa3-antibody-wb-transfected-hek293t-cells.jpg)
Western blot - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)All lanes : Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY ANXA3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 36 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)](https://www.abcam.com/ps/products/273/ab273649/Images/ab273649-394440-anti-anxa3-antibody-ihc-human-lung-tissue.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A3/ANXA3 antibody [OTI5G4] - BSA and Azide free (ab273649)Immunohistochemical analysis of paraffin-embedded human lung tissue staining of ANXA3 with a mouse monoclonal
Annexin A3/ANXA3 antibody [OTI5G4]This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.