Recombinant human Granulin protein (ab113403)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: DDDDK tag C-Terminus
Preparation and Storage
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Alternative names
- Acrogranin
- CLN11
- GEP
see all -
Function
Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. -
Tissue specificity
In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. -
Involvement in disease
Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. -
Sequence similarities
Belongs to the granulin family. -
Post-translational
modificationsGranulins are disulfide bridged. -
Cellular localization
Secreted. - Information by UniProt
Images
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Cellular activity - Recombinant human Granulin protein (ab113403)
Chemotaxis effect of Recombinant human Granulin protein in THP-1 Cells: Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24 well transwell polystyrene membrane with 8μm size pores. 0ng/ml, 10ng/ml, 100ng/ml and 250ng/ml of Granulin protein were diluted in RPMI 1640 supplemented with 0.5% FBS respectively. In case boiled Granulin protein were boiled at 100°C for 10min. And then Granulin protein samples were added to the lower chamber. THP-1 cells (2 X 105 cells per well), in the same media with Granulin protein, were added to the upper chamber. After 16 hours incubation at 37°C in 5% CO2 humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from triplicate wells were enumerated on a hemacytometer by means light microscopy.
