Mouse CD23 ELISA Kit (ab206984)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CD23 ELISA Kit
See all CD23 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 7.9% Inter-assay Sample n Mean SD CV% Serum 3 9.5% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 126.1 119.2% - 133.4% Cell culture media 120.7 117% - 123.3% EDTA Plasma 89.2 85.8% - 92.3% Cit plasma 81.3 77.8% - 83.9% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Pig -
Product overview
Mouse CD23 ELISA Kit (ab206984) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD23 protein in cell culture supernatant, serum, cit plasma, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD23 with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD23 is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. It has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). It is a 47 kDa type II transmembrane glycoprotein containing a short N-terminal cytoplasmic tail, a transmembrane domain, and extracellular domain containing C-type lectin domain. Two membrane isoforms and soluble CD23 fragements exist.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CD23 Capture Antibody 1 x 600µl 10X Mouse CD23 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse CD23 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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Alternative names
- Blast 2
- BLAST-2
- Blast2
see all -
Database links
- Entrez Gene: 14128 Mouse
- SwissProt: P20693 Mouse
Images
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Other - Mouse CD23 ELISA Kit (ab206984)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of CD23 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of CD23 standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of CD23 in mouse serum, plasma and cell culture supernatant samples.The concentrations of CD23 were measured in duplicates, interpolated from the CD23 standard curves and corrected for sample dilution. Note that 1X Diluted serum samples were pre-diluted to 2.5%. Note that 1X Diluted plasma samples were pre-diluted to 5%. Note that 1X Diluted Mouse spleen Day 5 cell culture supernatant samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are graphed in pg of CD23 per mL of neat sample (mean +/- SD, n=2).
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Sandwich ELISA - Mouse CD23 ELISA Kit (ab206984)To learn more about the advantages of recombinant antibodies see here.
