HRP Anti-Mitofusin 1 antibody [11E91H12] (ab198410)
![HRP Anti-Mitofusin 1 antibody [11E91H12] (ab198410)](https://www.abcam.com/ps/products/198/ab198410/Images/ab198410-244655-WBab1984101.jpg "HRP Anti-Mitofusin 1 antibody [11E91H12] (ab198410)")
Key features and details
- HRP Mouse monoclonal [11E91H12] to Mitofusin 1
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-Mitofusin 1 antibody [11E91H12]
See all Mitofusin 1 primary antibodies -
Description
HRP Mouse monoclonal [11E91H12] to Mitofusin 1 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment corresponding to Mouse Mitofusin 1.
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Positive control
- WB: NIH 3T3 whole cell lysate.
Properties
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Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
11E91H12 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-Mitofusin 1 antibody [11E91H12] (ab198410)HRP Anti-Mitofusin 1 antibody [11E91H12] (ab198410) at 1/5000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 40 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198410 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
