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Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16355] to Interferon alpha 6 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free
    See all Interferon alpha 6 primary antibodies

  • Description

    Rabbit monoclonal [EPR16355] to Interferon alpha 6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251131 is the carrier-free version of ab192264. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251131 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)
    Western blot - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)
    All lanes : Anti-Interferon alpha 6 antibody [EPR16355] (ab192264) at 1/5000 dilution

    Lane 1 : U937 (Human histiocytic lymphoma cell line) cell lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human fetal lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa



    This data was developed using ab192264, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)
    Immunocytochemistry - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)
    This data was developed using ab192264, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Interferon alpha 6 with ab192264 at 1/50 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue). The negative controls are as follows;
    1. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
    2. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
  • Flow Cytometry - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)
    Flow Cytometry - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)
    This data was developed using ab192264, the same antibody clone in a different buffer formulation.Flow cytometry analysis of Jurkat cells labelling Interferon alpha 6 (red) with purified ab192264 at dilution of 1/600. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)
    Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (ab251131)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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