Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227705 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227705 for 10 mins at room temperature.

Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling TMEM173 with purified ab227705 at 1/20 dilution (12.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227705 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).

Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.