Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMEM173 antibody [EPR13130] (ab181125)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13130] to STING
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-STING antibody [EPR13130]
    See all STING primary antibodies

  • Description

    Rabbit monoclonal [EPR13130] to STING

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • THP1 or HACAT lysate, THP1 or HACAT cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-STING antibody [EPR13130] (ab181125)
    Western blot - Anti-STING antibody [EPR13130] (ab181125)
    All lanes : Anti-STING antibody [EPR13130] (ab181125) at 1/10000 dilution (purified)

    Lane 1 : THP-1 cell lysate
    Lane 2 : HACAT cell lysate
    Lane 3 : human spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [EPR13130] (ab181125)
    Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [EPR13130] (ab181125)

    Immunocytochemistry/immunofluorescence analysis of THP-1 (human monocytic leukemia monocyte) cells labelling TMEM173 with ab181125 at 10 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
    Confocal image showing cytoplasmic staining in THP-1 cells.

  • Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)
    Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)

    Overlay histogram showing THP-1 cells fixed in 4% PFA and stained with purified ab181125 at a dilution of 1 in 20 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

    Alexa Fluor® 488 (ab198950) and R-PE (ab208874) conjugated versions are available for this clone.

  • Western blot - Anti-STING antibody [EPR13130] (ab181125)
    Western blot - Anti-STING antibody [EPR13130] (ab181125)
    Anti-STING antibody [EPR13130] (ab181125) at 1/2000 dilution (purified) + HEK293 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Observed band size: 37 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-STING antibody [EPR13130] (ab181125)
    Western blot - Anti-STING antibody [EPR13130] (ab181125)
    All lanes : Anti-STING antibody [EPR13130] (ab181125) at 1/2000 dilution (unpurified)

    Lane 1 : THP1 lysate
    Lane 2 : HACAT lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  • Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)
    Flow Cytometry - Anti-STING antibody [EPR13130] (ab181125)

    Flow cytometry analysis of THP1 cells using unpurified ab181125 at a 1/10 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Anti-STING antibody [EPR13130] (ab181125)
    Anti-STING antibody [EPR13130] (ab181125)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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