Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMEM173 antibody [EPR13130] - BSA and Azide free (ab227128)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13130] to STING - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-STING antibody [EPR13130] - BSA and Azide free
    See all STING primary antibodies

  • Description

    Rabbit monoclonal [EPR13130] to STING - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    Ab227128 is the carrier-free version of ab181125. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227128 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)

    Immunofluorescence staining of HeLa cells with purified ab181125 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab181125 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181125).

  • Flow Cytometry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Flow Cytometry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)

    Clone EPR13130 (ab227128) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM173 antibody [EPR13130] (PE). Please refer to ab208874 for protocol details.

    Overlay histogram showing HeLa cells stained with ab208874 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208874, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)

    Clone EPR13130 (ab227128) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM173 antibody [EPR13130] (Alexa Fluor® 647). Please refer to ab198952 for protocol details.

    ab198952 staining TMEM173 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198952 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLacells fixed with 4% formaldehyde (10 min).

  • Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)

    Clone EPR13130 (ab227128) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM173 antibody [EPR13130] (Alexa Fluor® 488). Please refer to ab198950 for protocol details.

    ab198950 staining TMEM173 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198950 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Flow Cytometry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Overlay histogram showing THP-1 cells fixed in 4% PFA and stained with purified ab181125 at a dilution of 1 in 20 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181125).

  • Flow Cytometry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Flow Cytometry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)

    Flow cytometry analysis of THP1 cells using unpurified ab181125 at a 1/10 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181125).

  • Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)

    Immunofluorescence analysis of HACAT cells (fixative 4% paraformaldehyde) labeling TMEM173 with unpurified ab181125 at a 1/100 dilution, and counterstained with DAPI. Goat anti rabbit IgG (Dylight® 555) secondary used at a 1/200 diution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181125).

  • Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Immunocytochemistry - Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)

    Immunofluorescence staining of HeLa cells with purified ab181125 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab181125 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181125).

  • Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)
    Anti-STING antibody [EPR13130] - BSA and Azide free (ab227128)

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