Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMEM173 antibody [SP338] - BSA and Azide free (ab238795)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP338] to STING - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-STING antibody [SP338] - BSA and Azide free
    See all STING primary antibodies

  • Description

    Rabbit monoclonal [SP338] to STING - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lymph node, thymus, tonsil, colon, spleen, colon adenocarcinoma, lung, lung squamous cell carcinoma, lung adenocarcinoma and pancreatic adenocarcinoma tissues. WB: THP-1 cell lysate. Flow Cyt: THP-1 cells. ICC/IF: THP-1 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab238795 is the carrier-free version of ab227704. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238795 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.

    The section was incubated with ab227704 for 10 mins at room temperature.

    This image was generated using ab227704, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227704 for 10 mins at room temperature. This image was generated using ab227704, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227704 for 10 mins at room temperature. This image was generated using ab227704, the same clone, but with a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Immunocytochemistry/ Immunofluorescence analysis of THP-1 (human monocytic leukemia monocyte) cells labeling TMEM173 with purified ab227704 at 1/50 (2.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated using ab227704, the same clone, but with a different buffer formulation.

  • Flow Cytometry - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Flow Cytometry - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Flow cytometry analysis of THP-1 (human monocytic leukemia monocyte) labeling TMEM173 with purified ab227704 at 1/20 dilution (5.25 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

    This image was generated using ab227704, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Flow Cytometry - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Flow Cytometry - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227704 at 1/400 dilution (green) compared with a rabbit IgG negative control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227704).

  • Anti-STING antibody [SP338] - BSA and Azide free (ab238795)
    Anti-STING antibody [SP338] - BSA and Azide free (ab238795)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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